MATrixAI Platform

AI-Assisted Customization of 3D-Printed Biomaterials

Interactive platform for ML-driven bioprinting parameter optimization

Model Accuracy
90%

Overall classification accuracy on test set

Dataset Size
150

Total bioprinting trials in dataset

Top Parameter
Cell Density

28% relative importance in model

Publications
7

Referenced research papers

Research Overview
AI-Assisted Customization of 3D-Printed Biomaterials for Tissue Engineering

3D bioprinting builds tissue-engineered constructs by depositing bioinks in precise geometries. However, balancing bioink formulation, extrusion settings, and cell viability is challenging: slight shifts in one parameter (e.g., viscosity, pressure, temperature) can drastically affect outcomes. Traditional trial-and-error approaches are laborious and low-throughput, often requiring dozens of manual experiments to identify workable settings for a single bioink–cell combination.

Machine learning offers a powerful solution by learning from a limited set of well-controlled experiments to predict outcomes across untested parameter combinations. This dashboard presents a supervised-learning framework for bioprinting optimization using a Random Forest classifier to predict cell viability categories.

Bioprinting Techniques
Comparison of major bioprinting approaches
Extrusion-based bioprinting

Extrusion-Based Bioprinting

A continuous filament of bioink is deposited layer by layer through a nozzle.

  • • Can handle high-viscosity inks and high cell densities
  • • Suitable for scaffolds with mechanical strength
  • • Lower resolution (~100 µm)
  • • Cells experience shear stress in the nozzle
Dataset Information
Experimental dataset details

Bioink Composition

2% w/v alginate, 5% w/v gelatin hydrogel

Sample Size

150 total prints (120 training, 30 testing)

Parameters Varied

  • • Nozzle temperature: 180–210 °C
  • • Print speed: 20–40 mm/s
  • • Cell density: 5–15 ×10⁶ cells/mL
  • • Viscosity: 2–4 Pa·s
  • • Layer height: 0.2–0.4 mm
  • • Crosslink time: 30–60 s

Evaluation Method

Live/dead fluorescent staining and structural integrity assessment

Future Directions

Closed-Loop Control

Equip bioprinters with real-time sensors and integrate defect-detection algorithms for automatic parameter adjustment.

In-Silico Exploration

Build hybrid simulators coupling fluid dynamics with ML surrogate models for virtual parameter testing.

Community Data Platform

Develop an open repository for labs to upload print settings and outcomes, enabling robust cross-material meta-models.